Application of the Indicator Amino Acid Oxidation Method for the Determination of the Metabolic Availability of Lysine from Indian Sorghum Protein in Young Adult Men (P08-069-19)
Bibliographic record
Abstract
To measure the protein quality of cooked sorghum by determining the metabolic availability (MA) of lysine; the first limiting indispensable amino acid. Additionally, we aimed to assess the effect of complementation by combining lentils and sorghum in a mixed meal format. In a repeated measures design, the MA of lysine in sorghum was studied using the indicator amino acid oxidation (IAAO) method with L-[1–13C] phenylalanine as the indicator at four levels of lysine: 5, 8, 12, 15 mg. kg−1. d−1 in five healthy adult men. These intakes represent 13, 21, 32 and 40%, respectively, of the lysine requirement (37 mg. kg−1. d−1) for adult men. Each subject participated in 8 experimental diets: 4 lysine intakes as L-lysine from crystalline amino acid, 3 intakes of lysine from sorghum prepared by moist cooking and 1 experiment to discern the effects of complementation with lentils. The principles of slope-ratio were applied to calculate the MA of lysine in sorghum by comparing the IAAO response to varying intakes of lysine in cooked sorghum relative to the IAAO response to free crystalline lysine in the reference protein which was modelled after egg protein. The MA of lysine from cooked sorghum was found to be 94%. Upon complementation with lentils, there was a decline in the oxidation of L-[1–13C] phenylalanine, reflecting an increased uptake of lysine for the synthesis of protein. The findings demonstrate that most of the lysine in cooked sorghum, the fifth most commonly consumed cereal grain, is available for protein synthesis despite being of limiting concentration. Upon complementation with lentils, there was a decrease in oxidation which highlights the effectiveness of complementation. A combination of sorghum and lentil protein in a 2:1 ratio is a viable strategy to improve the protein quality for individuals consuming a plant-based sorghum and lentil diet. Supported by the Canadian Institutes for Health Research. Pfizer Consumer Healthcare (Mississauga, Ontario) donated the multivitamins. Mead Johnson Nutritionals donated the protein-free powder for experimental diets.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".