Expression of Spaetzle Homologs 2-6 in Drosophila Larvae Hemocytes
Why this work is in the frame
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Bibliographic record
Abstract
In Drosophila, the gene spaetzle (Spz) encodes a protein involved in the production of an immune cell called a hemocyte. Specifically, this protein triggers a signal transduction cascade (a series of intracellular chain reactions) in order to produce the immune cell. However, there are 5 other Spz homologs (genes that are similar to one another), and little is known about their role in hemocyte production. My goal was to specifically examine the expression of each Spz homolog in hemocyte cells. Using RNA extracted from hemocytes of Drosophila larvae, a RT-PCR (a method of amplifying RNA via a DNA intermediate) was conducted using primers specific to each homolog, and the PCR products were then run out through gel electrophoresis. The bands produced on the gel were then compared to the expected cDNA sizes of each Spz homolog from flybase to determine if there was expression of the homologs in hemocytes. The Spz homologs 2, and 3 consistently showed expression in Drosophila larvae hemocytes, while the homologs Spz 4, Spz 5 and Spz 6 also showed expression. However, the expression seen with these three homologs was not as consistent compared to the others. Therefore, the homologs Spz 2 and Spz 3 are clearly expressed in Drosophila larvae hemocyte cells. Faculty Mentor: Agata Becalska Department: Biological Science
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it