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Record W2982181481

Expression of Spaetzle Homologs 2-6 in Drosophila Larvae Hemocytes

2019· article· en· W2982181481 on OpenAlexaff
Kyle Blacher

Bibliographic record

VenueMacEwan University Student Research Proceedings · 2019
Typearticle
Languageen
FieldImmunology and Microbiology
TopicInvertebrate Immune Response Mechanisms
Canadian institutionsMacEwan University
Fundersnot available
KeywordsBiologyGeneComplementary DNAHomologous chromosomeDrosophila melanogasterMolecular biologyGene expressionRNACell biologyDrosophila (subgenus)Genetics
DOInot available

Abstract

fetched live from OpenAlex

In Drosophila, the gene spaetzle (Spz) encodes a protein involved in the production of an immune cell called a hemocyte. Specifically, this protein triggers a signal transduction cascade (a series of intracellular chain reactions) in order to produce the immune cell. However, there are 5 other Spz homologs (genes that are similar to one another), and little is known about their role in hemocyte production. My goal was to specifically examine the expression of each Spz homolog in hemocyte cells. Using RNA extracted from hemocytes of Drosophila larvae, a RT-PCR (a method of amplifying RNA via a DNA intermediate) was conducted using primers specific to each homolog, and the PCR products were then run out through gel electrophoresis. The bands produced on the gel were then compared to the expected cDNA sizes of each Spz homolog from flybase to determine if there was expression of the homologs in hemocytes. The Spz homologs 2, and 3 consistently showed expression in Drosophila larvae hemocytes, while the homologs Spz 4, Spz 5 and Spz 6 also showed expression. However, the expression seen with these three homologs was not as consistent compared to the others. Therefore, the homologs Spz 2 and Spz 3 are clearly expressed in Drosophila larvae hemocyte cells.   Faculty Mentor: Agata Becalska Department: Biological Science

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.036
GPT teacher head0.303
Teacher spread0.267 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2019
Admission routes1
Has abstractyes

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