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Record W2997604664 · doi:10.22215/etd/2019-13850

Effects of Early-Life Stress on AMPA Receptors in the Auditory Cortex

2019· dissertation· en· W2997604664 on OpenAlexaff
Carinna Moyes

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldNeuroscience
TopicNeuroscience and Neuropharmacology Research
Canadian institutionsCarleton University
Fundersnot available
KeywordsAMPA receptorNeuroscienceBiologyGlutamate receptorPsychologyReceptorBiochemistry

Abstract

fetched live from OpenAlex

Critical period (CP) plasticity in the auditory cortex (A1) has been known to be crucial for both functional brain development and cognitive function.Impaired A1 development during a CP for tonotopic mapping has been implicated in many neurological disorders of learning and memory, including Autism.Our recent results have shown a critical role for α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPAR) in the auditory CP for tonotopic mapping.Here, we aim to determine if early-life stress (ELS) during rapid synaptic development affects the function of AMPARs required for normal CP plasticity.ELS was induced at P3-15 in a c-Fos based transgenic mouse model.Using whole-cell patch-clamp recordings, we recorded pyramidal cells in layer IV of A1 to measure AMPAR function and the maturation of glutamatergic synapses in P12-15 mice.We found that AMPAR functional maturation is highly correlated to the opening of A1 tonotopic CP plasticity during normal development.We further identified that ELS selectively activated a subpopulation of A1 pyramidal neurons as evidenced by selective activity-dependent green fluorescent protein tagging.Interestingly, while ELS did not cause significant changes in AMPAR function in overall randomly sampled neurons, ELS activated neurons showed enhancement of AMPAR function compared to non-activated neurons.These results provide a potential synaptic mechanism following exposure to a stressor during a CP of brain development and might identify novel strategies to modulate ELS-induced neurodevelopmental impacts.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.029
GPT teacher head0.348
Teacher spread0.319 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2019
Admission routes1
Has abstractyes

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