Genetic diversity of Plum pox virus in Ukraine
Bibliographic record
Abstract
Aim. Establishing the genetic diversity of Plum pox virus (PPV) in Ukraine. Methods. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), polymerase chain reaction with reverse transcription (RT-PCR), DNA sequencing and phylogenetic analysis. Results. The samples with visual symptoms of virus infection were collected from different regions of Ukraine and diagnosed using DAS-ELISA. The amplicons of the coat protein (CP) gene were obtained using RT-PCR and sequenced. The obtained viral cDNAs were analyzed using phylogenetic methods to elucidate the genetic diversity of PPV in different regions of Ukraine. The phylogenetic relationships between Ukrainian isolates were studied and compared to PPV isolates from neighboring countries. Conclusions. The PPV strains M and D circulate in Ukraine. For the first time, PPV was found in the Kharkiv region. The PPV isolates circulating in Ukraine are similar to the isolates from Germany, Turkey, Hungary, and Canada. The results can be used to predict the spread of the virus in different Ukrainian regions and in neighboring countries and to establish its origin and predict the development of possible epidemics.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".