Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris
Why this work is in the frame
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Bibliographic record
Abstract
Abstract In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4 , in rhamnose metabolism. P. pastoris GS115m/ LacB was subsequently constructed via introducing a β-galactosidase gene, LacB , under the control of rhamnose-inducible P LRA3 into P. pastoris GS115m. P. pastoris GS115m/ LacB greatly improved recombinant protein production relative to the parental strain ( P . pastoris GS115/ LacB ). In the present study, transcriptomes of P . pastoris GS115m/ LacB and P . pastoris GS115/ LacB grown in YPR medium were analyzed. P . pastoris GS115m/ LacB was found to suffer from the mild carbon source starvation. To attenuate the starvation stress, P . pastoris GS115m/ LacB attempted to enhance rhamnose metabolism by elevating the transcription levels of rhamnose-utilization genes LRA1 - 3 and RhaR . The transcription level of LacB under the control of P LRA3 thereby increased, resulting in the improved production of recombinant protein in P . pastoris GS115m/ LacB . It was also revealed that P . pastoris GS115m/ LacB cells coped with carbon starvation mostly via autophagy.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it