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Record W3012788229 · doi:10.7554/elife.55646

A roadmap for gene functional characterisation in crops with large genomes: Lessons from polyploid wheat

2020· article· en· W3012788229 on OpenAlexaff
Nikolai M. Adamski, Philippa Borrill, Jemima Brinton, Sophie A. Harrington, Clémence Marchal, Alison R. Bentley, William D. Bovill, Luigi Cattivelli, James Cockram, Bruno Contreras‐Moreira, Brett Ford, Sreya Ghosh, Wendy Harwood, Keywan Hassani‐Pak, Şadiye Hayta, Lee T. Hickey, K. Kanyuka, Julie King, Marco Maccaferrri, Guy Naamati, Curtis Pozniak, Ricardo H. Ramírez-González, Carolina Sansaloni, Ben Trevaskis, Luzie U. Wingen, Brande B. H. Wulff, Cristóbal Uauy

Bibliographic record

VenueeLife · 2020
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicWheat and Barley Genetics and Pathology
Canadian institutionsUniversity of Saskatchewan
FundersBiotechnology and Biological Sciences Research CouncilDirectorate for Biological SciencesJohn Innes FoundationRoyal SocietyEuropean Molecular Biology Laboratory
KeywordsPolyploidGenomeBiologyGeneGeneticsComputational biologyBiotechnologyEvolutionary biology

Abstract

fetched live from OpenAlex

Understanding the function of genes within staple crops will accelerate crop improvement by allowing targeted breeding approaches. Despite their importance, a lack of genomic information and resources has hindered the functional characterisation of genes in major crops. The recent release of high-quality reference sequences for these crops underpins a suite of genetic and genomic resources that support basic research and breeding. For wheat, these include gene model annotations, expression atlases and gene networks that provide information about putative function. Sequenced mutant populations, improved transformation protocols and structured natural populations provide rapid methods to study gene function directly. We highlight a case study exemplifying how to integrate these resources. This review provides a helpful guide for plant scientists, especially those expanding into crop research, to capitalise on the discoveries made in Arabidopsis and other plants. This will accelerate the improvement of crops of vital importance for food and nutrition security.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.927
Threshold uncertainty score0.467

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.231
Teacher spread0.186 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations111
Published2020
Admission routes1
Has abstractyes

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