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Record W3012899222 · doi:10.1094/phyto-12-19-0478-a

Genome Sequence of the Chestnut Blight Fungus <i>Cryphonectria parasitica</i> EP155: A Fundamental Resource for an Archetypical Invasive Plant Pathogen

2020· article· en· W3012899222 on OpenAlexafffund
Jo Anne Crouch, Angus L. Dawe, Andrea Aerts, Kerrie Barry, Alice C. L. Churchill, Jane Grimwood, Bradley I. Hillman, Michael G. Milgroom, Jasmyn Pangilinan, Myron L. Smith, Asaf Salamov, Jeremy Schmutz, Jagjit S. Yadav, Igor V. Grigoriev, Donald L. Nuss

Bibliographic record

VenuePhytopathology · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant and Fungal Interactions Research
Canadian institutionsCarleton University
FundersLos Alamos National LaboratoryLawrence Livermore National LaboratoryNational Institute of Food and AgricultureJoint Genome InstituteU.S. Department of AgricultureNatural Sciences and Engineering Research Council of CanadaNew Jersey Agricultural Experiment StationAgricultural Research ServiceUniversity of CincinnatiOffice of ScienceU.S. Department of EnergyNational Science Foundation
KeywordsCryphonectriaBiologyChestnut blightMycovirusGenomeFungusWhole genome sequencingSanger sequencingGeneticsShotgun sequencingPathogenBotanyDNA sequencingComputational biologyEvolutionary biologyGeneRNA

Abstract

fetched live from OpenAlex

Cryphonectria parasitica is the causal agent of chestnut blight, a fungal disease that almost entirely eliminated mature American chestnut from North America over a 50-year period. Here, we formally report the genome of C. parasitica EP155 using a Sanger shotgun sequencing approach. After finishing and integration with simple-sequence repeat markers, the assembly was 43.8 Mb in 26 scaffolds (L50 = 5; N50 = 4.0Mb). Eight chromosomes are predicted: five scaffolds have two telomeres and six scaffolds have one telomere sequence. In total, 11,609 gene models were predicted, of which 85% show similarities to other proteins. This genome resource has already increased the utility of a fundamental plant pathogen experimental system through new understanding of the fungal vegetative incompatibility system, with significant implications for enhancing mycovirus-based biological control.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.008
Threshold uncertainty score0.016

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.070
GPT teacher head0.310
Teacher spread0.239 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations50
Published2020
Admission routes2
Has abstractyes

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