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Record W3016819966 · doi:10.17975/sfj-2020-002

<i>PEX1</i> and <i>PEX7</i> Genes are Necessary for Completion of Immune Pathways and Survival Post-Infection

2020· article· en· W3016819966 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.
venuePublished in a venue whose home country is Canada.

Bibliographic record

VenueSTEM Fellowship Journal · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPeroxisome Proliferator-Activated Receptors
Canadian institutionsDalhousie University
FundersDalhousie University
KeywordsPeroxisomeImmune systemInnate immune systemBiologyImmunityCell biologyFunction (biology)Drosophila melanogasterBiogenesisOrganismGeneImmunologyGenetics

Abstract

fetched live from OpenAlex

The innate immune system consists of a variety of different immune pathways including the c-Fos and NF-κB immune pathways and is the first line of an organism’s defence against infection. This research analyzes the role of peroxisomes, an organelle present in eukaryotic cells, in the regulation of the c-Fos mediated pathway in innate immunity. Quantitative PCR was used to measure how reduced peroxisome function affected the relative expression of c-Fos in human fibroblasts. The effects of impaired peroxisome function on the overall ability of an organism to fight off infection were investigated by tracking Drosophila melanogaster survival post-infection with E. coli. In addition, the effects of peroxisomes on NF-κB regulated pathway were quantified in D. melanogaster fibroblasts by measuring the relative expression of NF-κB. Results showed that at least partial peroxisome function is necessary for the c-Fos and NF-κB mediated pathways and that without peroxisomes, D. melanogaster has a significantly reduced chance of survival post-infection. Interestingly, greater concentrations of fatty acids in the cell were shown to have an inhibitory effect on the cell’s ability to complete the immune response. Overall, this study provides evidence that peroxisomes are necessary for innate immune function. More extensive future research on peroxisome function could be used to applied to better understand and treat immune disorders.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.028
Threshold uncertainty score0.664

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.027
GPT teacher head0.235
Teacher spread0.208 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it