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The Role of Innate Immunity in Dystrophic Diaphragm Pathology

2020· article· en· W3017050097 on OpenAlexaffabout
Basil J. Petrof

Bibliographic record

VenueThe FASEB Journal · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMuscle Physiology and Disorders
Canadian institutionsMcGill University Health Centre
Fundersnot available
KeywordsInnate immune systemDystrophinDuchenne muscular dystrophyMedicineImmunologyChemokineMacrophageCCR2TLR4FibrosisMonocyteBiologyInflammationImmune systemChemokine receptorPathologyInternal medicineGenetics

Abstract

fetched live from OpenAlex

The pathophysiology of Duchenne muscular dystrophy (DMD) is complex and incompletely understood. However it is clear that the primary defect, lack of dystrophin, is necessary but not sufficient on its own to fully account for the onset of muscle fiber destruction, leading to eventual replacement by fibrotic tissue. Dystrophin restoration strategies are currently under clinical development, but the only treatment clearly shown to slow disease progression thus far in human DMD patients is the use of anti‐inflammatory corticosteroids. This presentation will focus on the role of the innate immune system in promoting the dysregulated inflammatory and aberrant muscle repair responses found in DMD. Using the mdx mouse diaphragm as a preclinical model due to its resemblance to the human DMD phenotype, the importance of monocyte‐derived macrophages in driving early disease progression will be outlined. This is exemplified by the therapeutic benefits (improved force generation and reduced fibrosis) noted when monocyte/macrophage recruitment to the dystrophic diaphragm is prevented through genetic or pharmacologic inhibition of the chemokine receptor CCR2. In addition, preventing activation of Toll‐like receptors (TLRs) is similarly beneficial and suggests significant involvement of damage‐associated molecular patterns (DAMPs) in DMD pathogenesis. Data will also be presented indicating that epigenetic alterations occur at the level of macrophage precursors in the bone marrow, which are likely stimulated by TLR4 recognition of DAMPs, and that subsequently play a role in dictating monocyte/macrophage phenotypic characteristics following their entry into the diseased muscles. Although the ideal treatment for DMD would be restoration of dystrophin to all muscles of the body including the diaphragm, significant hurdles remain for this ultimate therapy. In the meanwhile, there are serious drawbacks to the current practice of treating DMD children with corticosteroids, which have major side effects in DMD such as obesity, diabetes and osteoporosis. Future development of a more targeted therapeutic approach based on innate immune system modulation could represent an important advance in the field. Support or Funding Information Canadian Institutes of Health Research

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.218
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2020
Admission routes2
Has abstractyes

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