P754 Quantitation of cytokines in rabbits following tri-antigen vaccine cocktail immunization and<i>T. pallidum</i>challenge
Why this work is in the frame
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Bibliographic record
Abstract
<h3>Background</h3> Immunological analysis of primary and secondary syphilis in rabbits and humans suggests that T helper cells mount a vigorous interferon-γ-dominated immune response (T<sub>H</sub>1) to facilitate macrophage-mediated clearance of <i>T. pallidum</i>. In this study, we used quantitative reverse transcriptase (qRT)-PCR to evaluate post-challenge rabbit cytokine profiles of primary lesions in unimmunized rabbits and rabbits immunized with a tri-antigen vaccine cocktail. <h3>Methods</h3> Groups of 8 male New Zealand White rabbits were immunized with a trivalent recombinant antigen cocktail, (N-term of TprK + N- term of Tpr Subfamily I + Tp 0751, emulsified in either of two custom adjuvants containing Natural or Synthetic TLR4 agonists + a natural Mincle agonist). Unimmunized control and immunized animals were intradermally challenged with 10<sup>5</sup><i> T. pallidum</i> (Nichols) at each of 10 sites. Lesion biopsies were collected at days 2 and 21 post-challenge. Expression of IFN-γ, TGF-β, p40 IL-12/23, IL-4, IL-2, TNF-α, IL-10, IL-17A, IL-17F, and IL-22 was quantified by qRT-PCR using plasmids containing the target rabbit cytokines sequences, and expression levels were normalized to rabbit HPRT. <h3>Results</h3> At day 2, transcripts for IFN-γ, IL-2, IL-17A, IL-17F, and TNF-α were significantly upregulated in both immunized groups (P<0.01) compared to controls, a finding consistent with the development of clinical delayed type hypersensitivity and the induction of a T<sub>H</sub>1-type immune response at challenge sites. At day 21, the level of IFN-γ was lower in the Natural adjuvant group (P<0.02), compared to controls, consistent with enhanced treponemal clearance in that group. <h3>Conclusion</h3> In the development of a syphilis vaccine, it is important to determine correlates of protection to allow for assessment of the induction of a protective immune response. Our results demonstrate a robust immunization-induced T<sub>H</sub>1 and T<sub>H</sub>17 proinflammatory response in immunized rabbit groups, which suggests an effective level of resistance conferred by immunization with the trivalent protein vaccine. <h3>Disclosure</h3> No significant relationships.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it