P754 Quantitation of cytokines in rabbits following tri-antigen vaccine cocktail immunization and<i>T. pallidum</i>challenge
Bibliographic record
Abstract
<h3>Background</h3> Immunological analysis of primary and secondary syphilis in rabbits and humans suggests that T helper cells mount a vigorous interferon-γ-dominated immune response (T<sub>H</sub>1) to facilitate macrophage-mediated clearance of <i>T. pallidum</i>. In this study, we used quantitative reverse transcriptase (qRT)-PCR to evaluate post-challenge rabbit cytokine profiles of primary lesions in unimmunized rabbits and rabbits immunized with a tri-antigen vaccine cocktail. <h3>Methods</h3> Groups of 8 male New Zealand White rabbits were immunized with a trivalent recombinant antigen cocktail, (N-term of TprK + N- term of Tpr Subfamily I + Tp 0751, emulsified in either of two custom adjuvants containing Natural or Synthetic TLR4 agonists + a natural Mincle agonist). Unimmunized control and immunized animals were intradermally challenged with 10<sup>5</sup><i> T. pallidum</i> (Nichols) at each of 10 sites. Lesion biopsies were collected at days 2 and 21 post-challenge. Expression of IFN-γ, TGF-β, p40 IL-12/23, IL-4, IL-2, TNF-α, IL-10, IL-17A, IL-17F, and IL-22 was quantified by qRT-PCR using plasmids containing the target rabbit cytokines sequences, and expression levels were normalized to rabbit HPRT. <h3>Results</h3> At day 2, transcripts for IFN-γ, IL-2, IL-17A, IL-17F, and TNF-α were significantly upregulated in both immunized groups (P<0.01) compared to controls, a finding consistent with the development of clinical delayed type hypersensitivity and the induction of a T<sub>H</sub>1-type immune response at challenge sites. At day 21, the level of IFN-γ was lower in the Natural adjuvant group (P<0.02), compared to controls, consistent with enhanced treponemal clearance in that group. <h3>Conclusion</h3> In the development of a syphilis vaccine, it is important to determine correlates of protection to allow for assessment of the induction of a protective immune response. Our results demonstrate a robust immunization-induced T<sub>H</sub>1 and T<sub>H</sub>17 proinflammatory response in immunized rabbit groups, which suggests an effective level of resistance conferred by immunization with the trivalent protein vaccine. <h3>Disclosure</h3> No significant relationships.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".