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Record W3023519538 · doi:10.17504/protocols.io.w95fh86

Nsil1-Msp1 RAD-digest Detailed Protocol v2

2019· preprint· en· W3023519538 on OpenAlexaff
Ben Sutherland, Claire E Rycroft, Kristina M. Miller

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsUniversity of British ColumbiaFisheries and Oceans CanadaDalhousie University
Fundersnot available
KeywordsProtocol (science)GenotypingIon semiconductor sequencingComputational biologyDNA sequencingComputer scienceBiologyPhysicsGeneticsMedicineGenotypeGene

Abstract

fetched live from OpenAlex

This protocol comes with no guarantees, and is primarily used for the authors’ purposes. This protocol is an adaptation from the work in the following publications: Mascher M, Wu S, Amand PS, Stein N, Poland J (2013) Application of Genotyping-by-Sequencing on Semiconductor Sequencing Platforms: A Comparison of Genetic and Reference-Based Marker Ordering in Barley. PLoS ONE 8(10): e76925. https://doi.org/10.1371/journal.pone.0076925 Recknagel, H.,Jacobs, A.,Herzyk, P., &Elmer, K. R.(2015).Double‐digest RAD sequencing using ion proton semiconductor platform (ddRADseq‐ion) with nonmodel organisms.Molecular Ecology Resources,15,1316–1329.https://doi.org/10.1111/1755-0998.12406

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.005
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Protocol · Consensus signal: none
Teacher disagreement score0.118
Threshold uncertainty score0.396

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.005
Meta-epidemiology (narrow)0.0030.003
Meta-epidemiology (broad)0.0030.002
Bibliometrics0.0040.002
Science and technology studies0.0030.001
Scholarly communication0.0020.001
Open science0.0040.002
Research integrity0.0010.006
Insufficient payload (model declined to judge)0.1180.172

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.274
Teacher spread0.258 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreProtocol

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations1
Published2019
Admission routes1
Has abstractyes

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