Fluorescence Mapping of α-Tocopherol Redox Activity in Supported Lipid Bilayers
Bibliographic record
Abstract
Lipid membranes have gained wide attention as assembly scaffolds, owing to their ability to self-assemble into well-defined fluid structures, allowing the compartmentalization and direct assembly of components of interest in a fluid platform. Model lipid membranes offer the opportunity to mimic and study processes occurring across these fluid mosaics such as respiration, transport and signaling. Armed with a spectroelectrochemistry microscopy setup, in this study we have explored redox reactions within lipid membranes, proof of concept experiments intended to address the response of membrane embedded redox systems. Experimentally, a supported lipid bilayer was formed on top on an indium tin oxide (ITO)-coated glass. H4BPMHC, a redox sensitive fluorogenic probe bearing the chromanol moiety of α-tocopherol (Vitamin E), and a BODIPY tail segment was incorporated within the lipid membrane. The electrochemical and optical properties were next studied using our total internal reflection fluorescence (TIRF) microscopy based spectro-electrochemical (SEC) setup, that enables monitoring emission while minimizing background by virtue of sampling a small ~ 300 nm region above the glass water interface, while also controlling the redox potential at the basal leaflet of the lipid membrane. Our results are consistent with oxidation of the chromanol moiety of the probe into a chromanone moiety through an irreversible process that elicits fluorescence, and further thermally induced rearrangement of the newly formed chromanone into a chromoquinone. We will discuss the general methodology, results, and implications of the work in the context of electron transport through relay redox systems.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".