A Simple, Quantitative Peroxidase Assay Demonstrating Enzyme Inhibition with L-cysteine
Bibliographic record
Abstract
We present a simple enzyme assay using the robust horseradish peroxidase and the indicator guaiacol which, when oxidized to form tetraguaiacol, can be detected and quantified using a visible spectrophotometer at 470nm. This assay uses buffers (NaPO4), substrates (H2O2) and equipment already widely used and available in most first-year laboratories and is a cost-effective addition to current experiments. In addition to determining and comparing specific activity under a variety of traditional variables (pH, temperature) participants will use L-cysteine, reported in the literature to be a non-competitive inhibitor of peroxidase. Lcysteine demonstrates a unique inhibition pattern in our assay losing its inhibitory characteristics in a concentration and time dependent manner. This gives the instructor a range of options to demonstrate enzyme inhibition as well as an opportunity for investigations and self-directed experimentation by the students with a more challenging result to consider and analyze.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".