The impact of chronic unpredictable stress on cuprizone-induced demyelination and the occurrence of depressive-like behavior in C57BL/6 mice
Bibliographic record
Abstract
Objective To investigate whether cuprizone-induced demyelinating mice is more susceptible to developing depressive-like behaviors induced by chronic unpredictable stress (CUS), and to examine the underlying neuropathological changes. Methods C57BL/6 mice were randomly divided into four groups: control (n=10), stress (n=12), cuprizone (n=12) and cuprizone+ stress group (n=12). Mice in stress group were exposed to CUS for 3 weeks and mice in cuprizone group were fed with 0.3% cuprizone-containing food for 3 weeks. Mice in cuprizone+ stress group were exposed to both stress and cuprizone, and mice in control group were fed with normal food without exposure to stress. After 3 week exposure, they were subjected to behavioral tests including spontaneous activity, anxiety level and depressive-like behaviors and their brains were possessed for Western blot to test myelin marker MBP and astrocyte activation marker GFAP. Results CUS did not change the locomotor activity, anxiety levels and sucrose preference of cuprizone-induced demylinating mice, but did significantly increase their immobile time in forced swimming test (control (109.3±8.0)s, stress (111.3±21.8)s, cuprizone (90.5±8.9)s, cuprizone+ stress (155.0±9.1)s). Western blot revealed that CUS further decreased MBP expression (control (1.014±0.06), stress(1.088±0.104), cuprizone(0.436±0.071), cuproznie+ stress(0.150±0.041), (P<0.05)) and increased GFAP content (control (1.026±0.045), stress(0.846±0.078), cuprizone(1.736±0.215), cuprizone+ stress(2.428±0.314), (P<0.05)) in cuprizone-induced demyelinating mice. Conclusion Our findings suggest that demyelinating pathology increases individual susceptibility to chronic stress, which may induce depressive behaviors through damage of oligodendrocyte/myelin. Key words: Chronic unpredictable stress; Cuprizone; Demyelination; Myelin basic protein; Glial frillary acidic protein; Major depressive disorder
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".