Allograft islet cell transplantation and postoperatively modified Edmonton protocol for 3 cases of type 1 diabetes mellitus
Bibliographic record
Abstract
Objective To investigate the pancreatic islet cell isolation technology,quality standards,and safety and effectiveness of allograft islet cell transplantation and modified Edmonton protocol in the treatment of type 1 diabetes (T1DM).Method Three cases of T1DM received allograft islet cell transplantation.The pancreas was taken from adult organ donation after cardiac death donor.All pancreatic organs were digested by Liberase enzyme,COBE 2991 cell separator,and discontinuous density gradient purification,to obtain high purity and high activity of islet cells.All these procedures were conducted in our GMP facilities.After culture for 12 h,testing islets reached transplant standards.After the percutaneous puncture of hepatic portal vein trunk of the recipients,islet cells were transplanted evenly through the portal vein into the liver of recipients.After islet cell transplantation,a modified Edmonton immunosuppressive protocol containing antithymocyte globulin (ATG),tacrolimus and mycophenolate mofetil was used,and the changes in blood glucose,C peptide and glycate hemoglobin were monitored regularly during a follow-up period of one year.Result The transplanted islet cells of 3 recipients were 460 00(-505 200 IEQ,and islet purity and viability were 38.5％-49.7％ and 95％-97％ respectively.The stimulation index of human islet was 2.8-5.4.The glucose levels of 3 recipients in the normal diet were strictly controlled below 7.8 mmol/L.Two recipients remained insulin-independent after 3-5 months.The dosage of insulin was decreased by 60％ in 1 patient after 12 months.The levels of C-peptide and HbA1c were all within normal range and renal functions were improved.No complications related to islet infusion including hypoglycemia,adverse drug reactions,acute rejection and infection were observed.Conclusion Our isolation techniques and methods of islet cells are reliable.Allograft islet cell transplantation and the improved Edmonton protocol in the short-term for treatment of T1DM is safe and effective,but its long-term effect is undergoing to observe in the future. Key words: Pancreatic islet cells transplantation; Diabetes mellitus type 1; Immunosuppression
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".