Effect of enzyme immobilization and<i>in vitro</i>digestion on the immune-reactivity and sequence of IgE epitopes in egg white proteins
Bibliographic record
Abstract
The effects of hydrolysis by free and immobilized forms of Neutrase (FN, IN, respectively) and Thermolysin (FT, IT, respectively) and in vitro digestion on the degree of hydrolysis (DH) of egg white proteins, molecular weight distribution of peptides, immune-reactivity and IgE epitopes of egg white proteins were investigated. With FT and IT in the intestinal digests, the proteolysis followed by in vitro digestion produced peptides smaller than 10 kDa. Hydrolysis with the immobilized enzymes had a greater effect than the free enzymes on increasing surface hydrophobicity. The lowest IgE-binding capacity was observed for the intestinal digest of IT-derived hydrolysates (3.3 ± 1.9%). Compared to in vitro digestion, proteolysis showed a significant effect on the immune-reactivity reduction of egg white proteins. Liquid chromatography-tandem mass spectrometry data showed that the most resistant epitopes to enzymatic hydrolysis and in vitro digestion were in ovomucoid, where epitope fragments 1-10, 1-14, 1-20, 4-20, 11-20, 61-74, 71-75 and 101-105 remained intact. Overall, the IgE-binding capacities of egg white proteins were not completely removed after the enzymatic hydrolysis and in vitro digestion due to the presence of intact proteins such as lysozyme and also due to the several immunoreactive peptides derived from egg white proteins.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".