Genome-Wide Identification and Bioinformatics Analysis of HvBZR Gene Family in Barley
Bibliographic record
Abstract
BZR (Brassinazole resistant transcription factor) gene family is one specific transcription factor in plant and a critical factor in BRs(Brassinosteroid) transduction pathway, which play a vital regulational role in growth and development, phytohormone signal transduction and responding to abiotic stress. In this study, we conduct the identification of HvBZR gene family in barley by informatics analysis, and accomplished systemic analysis of HvBZR gene family with regard to gene structure, chromosome location, phylogenetic evolution, physicolchemical characteristic, protein conserve motif and transcriptome data analysis and so on. The result of genome wide identification reveals that HvBZR gene family consisted of seven members, which is unevenly distributed in the different chromosomes; the result of gene structure analysis reflects that the length of gene and numbers of introns varied obliviously among the HvBZR gene family members; the protein sequence analysis of physicolchemical characteristic, secondary structure and three dimensional structure reflect that they possessed the similarity; the subcellular location of HvBZRs is distributed in nucleus; the conserved motif analysis of HvBZRs reveals that all HvBZRs contain (pf8657) conserved domain and partial members harboured another domain(pf01373); phylogenetic evolution analysis demonstrated the relationship is closer between the barley and the rice; a lot of cis-element is distributed in the putative promoter region, which is related to growth and development and hormone signal transduction and abiotic stress resoponsiveness based on the analysis of the putative promoter sequence; the expression profiles is distrinct obviously in different tissue and organ during the different development stage based on the transcriptome data. In this research, we conducted the systemic and detailed analysis of HvBZR gene family, in order to providing some theoretical basis for the analysis of the biological function of BZR transcription factors in barley.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".