MétaCan
Menu
Back to cohort
Record W3041554972

Hypoxia and the metabolic phenotype of Daphnia

2020· dissertation· en· W3041554972 on OpenAlexfundno aff
K. Westbury

Bibliographic record

VenueQSpace (Queen's University Library) · 2020
Typedissertation
Languageen
FieldPharmacology, Toxicology and Pharmaceutics
TopicChemical Reactions and Isotopes
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsHypoxia (environmental)PhenotypeDaphniaBiologyZoologyGeneticsChemistryGeneOxygenCrustacean
DOInot available

Abstract

fetched live from OpenAlex

Environmental hypoxia is a phenomenon in which low oxygen conditions force organisms to respond behaviourally and physiologically to survive in an otherwise lethal habitat.Animals display different mechanisms to cope with the harsh living conditions of a hypoxic environment, most of which can be summarized as: (i) improvement of oxygen transport capacity, (ii) improvement of oxygen storage, and (iii) supply of energy via anaerobic metabolism.Daphnia respond to hypoxia by increasing the expression of extracellular hemoglobin (Hb), an oxygen transport/storage protein.It is generally thought that this upregulation is used as a strategy to sustain sufficient oxygenation to tissues, allowing Daphnia to stay systemically normoxic even when the environment is hypoxic.Although the changes/differences in Hb levels have been studied in many contexts, very little has been done exploring the basic properties of energy metabolism in Daphnia.My focus was on the link between the Hb response and the metabolic phenotype.I sampled different Daphnia species from 20 lakes in the Frontenac Arch Biosphere.The resulting metabolite analyses support the idea that the benefit of Hb is facilitating oxygen delivery rather than to increase oxygen storage.I found a distinct lack of coordination in the responses of the glycolytic enzymes relative to Hb. Daphnia pulicaria from four lakes appeared to demonstrate one of two strategies in relation to coordination of glycolytic genes and Hb.In some lakes D. pulicaria showed a pronounced induction of Hb without changes in glycolytic enzymes, whereas other lakes showed a blunted Hb response but pronounced glycolytic response.Serial sampling showed the Hb response early in the season (May-June) and a glycolytic response later in the season (September-October).Further studies should include transcriptome analyses exploring if lake-by-lake differences are due to microevolutionary variation or phenotypic responses to different degrees and durations of oxygen stress.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.276
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.024
GPT teacher head0.289
Teacher spread0.265 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designNot applicable
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2020
Admission routes1
Has abstractyes

Explore more

Same venueQSpace (Queen's University Library)Same topicChemical Reactions and IsotopesFrench-language works237,207