A PCR/LDR-Based Functional Molecular Marker of Rice ( Oryza Sativa L. ) Aroma Allele BADH2-E2
Bibliographic record
Abstract
The loss-of-function of betaine aldehyde dehydrogenase 2 (BADH2) in rice leading to the accumulation of 2-acetyl-1-pyrrolidine (2AP), the substrate of enzymatic reaction , makes the rice flavor. The rice aroma allele BADH2-E2 has been widely used in aroma rice breeding in China. In this study, a PCR/LDR-based functional molecular marker was developed according to the 7bp deletion in the second exon of badH2-E2 aroma allele. Using PCR/LDR-based markers, the genotypes of Shenfan24, Shenfan26 and their F1 hybrids in the badH2-E2 locus could be accurately identified, and screening the F2 population derived from the cross of Shenfan24 and Shenfan26 shows that the segregation ratio of badH2-E2 positive homozygous, heterozygous and badH2-E2 negative homozygous were 1:2:1. The results of this study provide a new type of molecular marker for marker-assisted breeding and have certain application potential.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".