Protocol for Primary Mouse Hepatocyte Isolation
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.
Machine scores (provisional)
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
- Teacher spread
- 0.284 · how far apart the two teachers sit on this one work
- Validation status
score_only:v0-immature-baseline· verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it
Abstract
Primary hepatocytes are a vital tool in various biomedical research disciplines, serving as an ex vivo model for liver physiology. Obtaining high yields of viable primary mouse hepatocytes is technically challenging, limiting their use. Here, we present an improved protocol based on the classic two-step collagenase perfusion technique. The liver is washed by perfusion, hepatocytes are dissociated by collagenase, separated from other cells, and cultured. This protocol was optimized to significantly reduce procedure duration and improve hepatocyte yield and viability.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- STAR Protocols
- Topic
- Liver physiology and pathology
- Field
- Medicine
- Canadian institutions
- —
- Funders
- Canadian Institutes of Health ResearchAzrieli FoundationIsrael Science FoundationAbisch-Frenkel-StiftungInternational Development Research Centre
- Keywords
- CollagenaseHepatocyteLiver perfusionPerfusionEx vivoIn vivoLimitingProtocol (science)Cell biologyIsolation (microbiology)Liver tissueChemistryBiologyMedicineBioinformaticsBiochemistryPathologyIn vitroInternal medicineEnzymeBiotechnologyEngineering
- Has abstract in OpenAlex
- yes