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Record W3082632815 · doi:10.1101/2020.08.31.274589

Human T-bet governs innate and innate-like adaptive IFN-γ immunity against mycobacteria

2020· preprint· en· W3082632815 on OpenAlexafffund
Rui Yang, Federico Mele, Lisa Worley, David Langlais, Jérémie Rosain, Ibithal Benhsaien, Houda Elarabi, Carys A. Croft, Jean‐Marc Doisne, Peng Zhang, Marc Weißhaar, David Jarrossay, Daniela Latorre, Yichao Shen, Jing Han, Conor Gruber, Janet Markle, Fatima Al Ali, Mahbuba Rahman, Taushif Khan, Yoann Seeleuthner, Gaspard Kerner, Lucas Husquin, Julia L. Maclsaac, Mohamed Jeljeli, Fatima Ailal, Michael S. Kobor, Carmen Oleaga‐Quintas, Manon Roynard, Mathieu Bourgey, Jamila El Baghdadi, Stéphanie Boisson‐Dupuis, Anne Puel, Frédéric Batteux, Flore Rozenberg, Nico Marr, Qiang Pan‐Hammarström, Dusan Bogunovic, Lluís Quintana‐Murci, Thomas Carroll, S. Cindy, Laurent Abel, Aziz Bousfiha, James P. Di Santo, Laurie H. Glimcher, Philippe Gros, Stuart G. Tangye, Federica Sallusto, Jacinta Bustamante, Jean‐Laurent Casanova

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2020
Typepreprint
Languageen
FieldImmunology and Microbiology
TopicImmune Cell Function and Interaction
Canadian institutionsOntario GenomicsBC Children's HospitalUniversity of British ColumbiaMcGill UniversityMcGill Genome Centre
FundersNew South Wales GovernmentHelmut Horten StiftungFonds de Recherche en Santé RespiratoireNational Institute of Allergy and Infectious DiseasesMedical Research CouncilUniversité Paris DescartesInstitut National de la Santé et de la Recherche MédicaleNational Center for Advancing Translational SciencesAgence Nationale de la RechercheNational Science FoundationStony Wold-Herbert FundSt. Giles FoundationGenome CanadaQatar National Research FundFondation pour la Recherche MédicaleImmune Deficiency FoundationFonds National de la Recherche LuxembourgNational Institutes of HealthSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungNational Health and Medical Research CouncilSidra MedicineBrigham and Women's Hospital
KeywordsImmunologyInnate immune systemAcquired immune systemBiologyImmunityAntigenInterferon gammaInterferonCytokineImmune system

Abstract

fetched live from OpenAlex

Summary Inborn errors of human IFN-γ immunity underlie mycobacterial disease. We report a patient with mycobacterial disease due to an inherited deficiency of the transcription factor T-bet. This deficiency abolishes the expression of T-bet target genes, including IFNG , by altering chromatin accessibility and DNA methylation in CD4 + T cells. The patient has profoundly diminished counts of mycobacterial-reactive circulating NK, invariant NKT (iNKT), mucosal-associated invariant T (MAIT), and Vδ2 + γδ T lymphocytes, and of non-mycobacterial-reactive classic T H 1 lymphocytes, the remainders of which also produce abnormally low amounts of IFN-γ. Other IFN-γ-producing lymphocyte subsets however develop normally, but with low levels of IFN-γ production, with exception of Vδ2 − γδ T lymphocytes, which produce normal amounts of IFN-γ in response to non-mycobacterial stimulation, and non-classic T H 1 (T H 1*) lymphocytes, which produce IFN-γ normally in response to mycobacterial antigens. Human T-bet deficiency thus underlies mycobacterial disease by preventing the development of, and IFN-γ production by, innate (NK) and innate-like adaptive lymphocytes (iNKT, MAIT, and Vδ2 + γδ T cells), with mycobacterial-specific, IFN-γ-producing, purely adaptive αβ T H 1* cells unable to compensate for this deficit.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.220
Teacher spread0.202 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations9
Published2020
Admission routes2
Has abstractyes

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