Analysis of Variation in <i>MYB1</i> and <i>MYB10</i> in Several <i>Malus</i> Plants
Bibliographic record
Abstract
For the study of the polymorphism and evolutionary characteristics of MYB1 and its allele MYB10 involved in the regulation of anthocyanin biosynthesis in genus Malus . The MYB1 and MYB10 gene sequences amplified by RT-PCR technology in several Malus plants were sequenced and predicted for their protein structures to detect the differences. Six MYB1 sequences were obtained and the full-length CDS were 1 239 bp except for those of Malus cv. Eleyi and Malus sieversii (1 209 bp). While nine MYB10 were sequenced and the full-length CDS were 732 bp ( Malus micromalus was 717 bp). The result from the comparison of protein sequences of MYB1 and MYB10 showed that the polymorphism of MYB1 was significantly higher than MYB10 and the polymorphism difference between the alleles may be related to the differentiation of gene function. Two polymorphism sites, the interchange of alkaline arginine and non-polar leucine, located on functional domain which may affect the function of MYB1 in different Malus plants. The result suggested that sequence conservatism of the allele MYB10 and MYB1 significantly differed from each other, and the genetic function responsible for evolution was differentiated. The result can provide further theoretical basis for the study of the gene function and regulation mechanism of apple fruit color.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".