DNA-barcoding identification of <i>Dothistroma septosporum</i> on <i>Pinus contorta</i> var. <i>latifolia, P. banksiana</i> and their hybrid in northern Alberta, Canada
Bibliographic record
Abstract
The ascomycete fungi Dothistroma septosporum (Dorogin) M. Morelet and D. pini Hulbary are both responsible for Dothistroma needle blight disease (DNB) on pines. Using a DNA-barcoding approach, we confirmed that the DNB infection of lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.), jack pine (P. banksiana Lamb.) and their hybrid in four research sites in northern Alberta (Canada) was caused by D. septosporum, confirming that the range of D. septosporum extends east of the Rocky Mountains into Alberta. Analysis of mating-type (MAT) genes showed the presence of both mating types in each of the pine plantations, indicating that the pathogen has the potential to complete its lifecycle in this region. Controlled inoculation confirmed that the two native pines found in this area (P. contorta var. latifolia and P. banksiana) and their hybrid are susceptible to D. septosporum, suggesting that continuous monitoring of the disease needs to be carried out to prevent any future outbreak in natural stands.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".