PSVI-31 The Effect of Aging on Peripheral Blood Mononuclear Cell Proliferation in Labrador Retrievers
Bibliographic record
Abstract
Abstract It is well documented that companion animals are living longer and as animals age their cells undergo immuno-aging or senescence, an age associated process of deterioration. In an effort to see how aging plays a role in immune cell proliferation, twelve healthy Labrador Retrievers (6-Young, 3Male/3Female, 6-Senior, 3Male/3Female) were selected to participate in a trial to determine what effect aging might have on the proliferative rate of peripheral blood mononuclear cells (PBMC). Mean age for Senior group was 8.74yrs, Young 2.38yrs (P.< 0.0001). Peripheral blood mononuclear cells (PBMC) were isolated, counted, seeded at a density of 5x104 in 96-well MP, cultured in RPMI-1640 complete medium (5% FBS), and mitogenic stimulation via 2-fold serial dilutions of Phytolacca Americana lectin (pokeweed mitogen-PWM) ranging from 0.8 -0.025 µg/mL. Following 48hr incubation in 5% CO2/95% humidity, 100µM Bromodeoxyuridine (5-bromo-2’-deoxyuridine, BrdU; a synthetic analog nucleoside of thymidine), was added to each culture well and further incubated 24hrs to allow DNA incorporation. Cells were harvested, denatured, and BrdU incorporated in newly synthesized DNA was bound by monoclonal antibodies (anti-BrdU-POD). The immune complexes were detected by tetramethyl-benzidine (TMB), reaction stopped with 1M Sulfuric acid (H2SO4), and quantified by measuring absorbance at 450nm. Proliferation was higher in Young dogs than Senior dogs at every concentration, signifying overall statistical significance (Mean: Young-0.972, Senior-0.697±0.057 SEM, P = 0.001). Rate difference by concentration was as follows: PWM 0.00 µg/mL: Young-0.109, Senior-0.194±0.031 (P = 0.099); 0.025 µg/mL: Young-0.788, Senior-0.481±0.201 (P = 0.321); 0.05 µg/mL: Young-1.002, Senior-0.610±0.207 (P = 0.227); 0.1 µg/mL: Young-1.113, Senior-0.776±0.195 (P = 0.244); 0.2 µg/mL: Young-1.249, Senior-0.915±0.172 (P = 0.217); 0.4 µg/mL: Young-1.271, Senior-0.965±0.148 (P = 0.191); 0.8 µg/mL: Young-1.252, Senior-0.936±0.117 (P = 0.101). These results coincide with other published literature, and these data suggest age plays a critical role in immunity and proliferative ability of PBMCs in the Labrador Retriever.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".