The “Rapid’Salmonella” Method: Estimation of the Limit of Detection for Salmonella Strains Typhimurium and Enteritidis Isolated from Frozen Poultry Meat
Bibliographic record
Abstract
As a part of evaluation the surveillance system of Salmonella in frozen imported poultry meat into Jordan, we conducted a study to estimate the limit of detection (LOD50% and LOD95%) of S. Typhimurium and S. Enteritidis based on chromogenic media of Rapid’Salmonella method. Salmonella-free chicken meat samples was inoculated with 1 to 100 CFU of 11 wild strains that originated from frozen imported poultry meat and 2 reference strains. In the experiment, the observed lowest concentration for Salmonella Typhimurium and Salmonella Enteritidis using Rapid’Salmonella method were from 1 to 50 CFU/25 g. Based on these results, probability of detection (POD) curve was estimated according to the model described in EN ISO 16140-4. From the estimated POD functions, the LOD50% and LOD95% was determined for the Rapid’Salmonella method. The LOD50% of the different strains varied from 0.9 to 21.2 CFU/25 g. The two reference strains and 9 wild strains had a LOD50% less than 2 CFU/25 g, one wild strain of Salmonella Enteritidis had a LOD50% of 6.8 CFU/25 g and another one had a LOD50% of 21.2 CFU/25 g. The majority of Salmonella strains has a LOD50% of 1-4 CFU/25 g in poultry meat, but also that there are some Salmonella strains which will first be detected at 10 CFU/25 g and higher.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".