Cytotoxic Effect of Nefazodone on HepG2 Cell Line and the Reliability of Comet Assay to Determine Genotoxicity.
Bibliographic record
Abstract
Nefazodone is atypical antidepressant which was manufactured by Bristol-Myers Squibb in 1994 to avoid the adverse effects associated with other antidepressants, including nausea, sedation, insomnia, cardiovascular toxi-city, weight gain and dysfunction. In 2004, nefazodone was withdrawn from USA after its withdrawal from Canada and Europe due to the reports of liver injury in patients treated with this drug. The current study was performed to investigate the cytotoxic and genotoxic effect of nefazodone on HepG2 cell line at different concentrations by using MTT assay and comet assay, respectively. The results showed that nefazodone causes a reduction in cells viability of HepG2 cell line with an IC50 4.682 µg/ml. Comet assay showed a significant increment in the three parameters (tail length, percent of DNA in tail and tail moment) in a concentration-dependent manner, when compared with negative control (p˂0.01), but these results considered as false positive due to cells death.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.013 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".