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Record W3110643032 · doi:10.1139/cjb-2020-0080

Vacuolar compartmentalisation and efflux of cadmium in barley

2020· article· en· W3110643032 on OpenAlexvenueno aff
Merrine Thomas, Robert J. Reid

Bibliographic record

VenueBotany · 2020
Typearticle
Languageen
FieldEnvironmental Science
TopicHeavy metals in environment
Canadian institutionsnot available
Fundersnot available
KeywordsVacuoleCadmiumGlutathioneEffluxBiologyBiochemistryTransporterAdenosine triphosphateDivalentPhytochelatinIon transporterATP-binding cassette transporterCell biologyChemistryEnzymeCytoplasmMembrane

Abstract

fetched live from OpenAlex

Plants have developed different tolerance strategies to deal with toxic metals. Various factors and mechanisms related to the compartmentalisation of cadmium (Cd) into the vacuoles and the efflux of Cd were investigated to analyse their roles in Cd tolerance in barley. In vacuolar transport assays, the addition of adenosine triphosphate (ATP) alone significantly increased Cd uptake into the vacuole, but more so when both ATP and glutathione (GSH) were supplied together. This suggests that Cd may be partly sequestered as Cd2+ ions via divalent cation transporters, but predominantly as Cd–GSH complexes, most likely via ATP-binding cassette transporter-type transporters. Comparison of the concentrations of 109Cd in whole protoplasts and vacuoles isolated from shoots demonstrated that the majority of the cellular Cd was located in the vacuole. Over 48 h, 12% of the root Cd was effluxed into the external solution when roots were loaded with 109Cd by foliar application. This active excretion may be a detoxification strategy, in addition to the compartmentalisation of the majority of cellular cadmium into vacuoles predominantly as complexed form.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.226
Teacher spread0.208 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations17
Published2020
Admission routes1
Has abstractyes

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