The Second Stalk of the FoF1‐ATP synthase
Bibliographic record
Abstract
The detailed structure and the protein‐protein interactions of the stator subunits of the F1Fo‐ATP synthase remain one of the important questions in the ATP synthase field since these interactions are directly related to the mechanics of energy conservation and storage of rotational energy during catalytic turnover. Understanding the structures and interactions is therefore crucial to the understanding of the overall catalytic mechanism of the synthase and especially that of energy transduction within the protein. We have used ESR and site‐specific spin‐labeling to gain further insight into the quaternary structure of the dimer formed by the subunits b of the Fo‐sub‐complex of the FoF1‐ATPsynthase. We introduced at total of 37 individual cysteine mutations into a water‐soluble mutant of the b‐dimer and have specifically spin‐labeled them with different cysteine‐specific spin‐labels. The distances between the spin‐labels within the b‐dimer were determined using low‐temperature ESR. We are using the distance constraints we obtained from ESR spectroscopy and molecular modeling and molecular dynamics techniques to determine the quaternary structure interactions of the dimer of b‐subunits. The data obtained by ESR will be compared to di‐sulfide cross linking pattern of the proteins.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".