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Record W3115327895 · doi:10.1101/2020.12.22.424010

Unearthing the genes of plant-beneficial marine yeast - <i>Wickerhamomyces anomalus</i> strain MSD1

2020· preprint· en· W3115327895 on OpenAlexaff
Subramanian Radhesh Krishnan, N. Prabhakaran, K. Sengali Ragunath, R. Srinivasan, K. Keerthana Ponni, G. Balaji, M. Gracy, Chinnasamy Brindha, Lakshmi Narayanan, K. Lavanya Latha

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2020
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicStudies on Chitinases and Chitosanases
Canadian institutionsStantec (Canada)
FundersBiotechnology Industry Research Assistance CouncilDepartment of Biotechnology, Ministry of Science and Technology, India
KeywordsGeneGenomeBiologyBiochemistryYeastORFSGeneticsPeptide sequenceOpen reading frame

Abstract

fetched live from OpenAlex

Abstract The de novo genome of unique marine yeast, Wickerhamomyces anomalus isolated from seaweed along Indian coast is presented. The genome assembly was carried out using MaSurCA assembler that generated a data size ∼14.3 mb from short and long reads obtained from Illumina Hiseq 4000 and GridION-X5 respectively. This assembled genome data were used for predicting genes using Augustus gene prediction tool that reported 6720 genes and proteins. The gene sequences were used to unravel the metabolic pathway analysis using KAAS database. The protein sequences were used for secondary analysis to predict the presence of signal peptides using SignalP tool, predicting protein family, domains using Pfam tool and prediction of transmembrane helices in proteins using TMHMM tool. Presence of genes involved in plant growth–promotion and regulation (PGPR) including siderophore and IAA production, iron and sulfur transformation, zinc and phosphate solubilization, nitrogen fixation, synthesis of anti-bacterial and volatile organic compound (VOCs), were assigned. Additionally, acid and alkaline phosphatases, ACC deaminases and lytic enzymes such as β-glucanases, proteases and chitinases involved in pathogen suppression, are also reported. The study elucidates comprehensive understanding of PGP attributes of MSD1 and its potential use in agriculture as bio-fertilizer /bio-stimulant.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.108
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.210
Teacher spread0.197 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations6
Published2020
Admission routes1
Has abstractyes

Explore more

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