Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype
Bibliographic record
Abstract
Since the 19th century, the presentation of bovines with disproportionate muscle development have been associated with mutations that inhibit the action of the myostatin gene, it is referred to as double muscle mutation, which is common in some European Bos taurus breeds but it is not reported in buffaloes Bubalus bubalis. This study aims to evaluate if the phenotype observed in 6 young buffaloes with disproportionate muscle development has the same myostatin mutation reported in cattle. DNA was obtained from the blood of the animals of the Murrah breed. First, second and third exon was amplified end point PCR; the fragments were sequenced using capillary electrophoresis. Holstein cattle (Bos taurus) was used As control for normal phenotype. The results obtained from the comparison of the sequence of the myostatin gene show that the observed double-muscled phenotype did not show differences from normal controls. Interspecific variation was demonstrated by comparing exons two and three of the gene, finding 12 variations between the Bos taurus and Bubalus bubalis species in the evaluated fragments. It is necessary to study physiology, and the animals to explain the phenotype observed in buffaloes.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".