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Record W3134453464

Chondroitin Sulphate Acts as an 'Alarm Cue' in Zebrafish

2020· article· en· W3134453464 on OpenAlexaffabout
Jasmin Bajwa

Bibliographic record

VenueMacEwan University Student Research Proceedings · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicZebrafish Biomedical Research Applications
Canadian institutionsMacEwan University
Fundersnot available
KeywordsThigmotaxisZebrafishALARMFish <Actinopterygii>ChemistryArtificial intelligenceOpen fieldComputer scienceFisheryBiologyBiochemistryEndocrinologyEngineering
DOInot available

Abstract

fetched live from OpenAlex

Zebrafish are conspecific organisms that are sensitive to the use of ‘alarm cues’, a procedure in behavioural fish research used to create experimental stress responses. We used a commercially available chemical compound, chondroitin sulphate, extracted from shark cartilage, as the possible ‘alarm cue’. This chemical compound controls for standardization errors common with alarm cues extracted from individual fish. We conducted an open field test, followed by a novel object approach test, to assess the behavioural responses consequential of 30 minutes of chondroitin sulphate exposure at different concentrations (ie. 1, 10, and 100 mg/L). Secondly, we locally applied 250 μL of 100 mg/L of chondroitin sulphate to the centre of an open field arena, and again assessed behavioural responses. The time individual fish spent in the centre, transition, and thigmotaxis zones was examined, along with the distance each fish moved and the time spent immobile. There was a significant increase in the time fish spent in the thigmotaxis zone during the novel object approach test after chondroitin sulphate exposure. We also found that fish in the experimental group spent more time in the thigmotaxis zone following the injection of chondroitin sulphate, compared to fish in the control group. These findings suggest this compound can be used as a reliable alarm cue stimulus in zebrafish.  Presented in absentia on April 27, 2020 at Student Research Day at MacEwan University in Edmonton, Alberta. (Conference cancelled) Faculty Mentor: Trevor Hamilton Department: Psychology NOTE: This work is available to MacEwan users only at https://roam.macewan.ca/islandora/object/gm:2042

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.007
Threshold uncertainty score0.014

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.064
GPT teacher head0.376
Teacher spread0.313 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2020
Admission routes2
Has abstractyes

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