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Record W3135875599 · doi:10.35334/harpodon.v13i2.1658

AMPLIFIKASI GENE mtDNA CO1 IKAN MALONG (MURAENESOCIDAE) DARI PERAIRAN KOTA TARAKAN DENGAN TEKNIK PCR

2021· article· id· W3135875599 on OpenAlexaboutno aff
Sumarlin Sumarlin, Clara Yovilan Moq, Syamsidar Gaffar, M. Gandri Haryono

Bibliographic record

VenueJurnal Harpodon Borneo · 2021
Typearticle
Languageid
FieldBiochemistry, Genetics and Molecular Biology
TopicIdentification and Quantification in Food
Canadian institutionsnot available
Fundersnot available
KeywordsMitochondrial DNABiologyMolecular biologyGeneGenetics

Abstract

fetched live from OpenAlex

Ikan Malong atau di Tarakan lebih dikenal dengan sebutan Ikan Ose merupakan ikan komersil yang tersebar secara luas di laut Indo-west pacific meliputi laut merah, teluk Persia, pantai barat India, dan sri langka hingga Fiji dan Tuvalu, bagian utara jepang dan korea, bagian selatan laut arafura, dan utara Australia. Di Indonesia Ikan Malong tersebar dari pulau Sumatra, Kalimantan, dan Sulawesi. Dengan penyebaran yang begitu luas sayangnya informasi genetik ikan Ose masih sangat terbatas. Penelitian ini dilakukan untuk mengisolasi dan mengamplifikasi gen mtDNA CO1 ikan ose dari perairan Kota Tarakan. Metode penelitian yang dilakukan meliputi isolasi genom mtDNA CO1 dengan mengikuti petunjuk Quick-DNA Tissue/Insect Miniprep Kit (Zymo Research, D6016). Dilanjutkan dengan amplifikasi (perbanyakan) untai gen mtDNA CO1 menggunakan 4 primer cocktail berdasarkan Canadian Center for DNA Barcoding (CCDB), 2006. Kemudian visualisasi produk PCR dengan menggunakan teknik gel elektroforesis. Terdapat dua jenis ikan ose yang dijadikan sampel pada penelitian ini, yaitu Ikan ose dengan perut berwarna kuning (Ose Kuning/Ose-K) dan Ikan ose dengan perut berwarna hitam ( Ose Hitam/Ose-H). Hasil isolasi dan pemurnian genom mtDNA CO1 merujuk pada rasio A260/280. Ekstrak gen mtDNA Ose-K memiliki konsentrasi 67.3 ng/µl dengan nilai rasio A260/280 diperoleh 1.94, dan konsentrasi untuk ekstrak gen mtDNA sampel Ose-H diperoleh 84.5 ng/µl dan 1.97 untuk nilai rasio A260/280. Hasil visualisasi produk PCR setelah di running pada gel elektroforesis diperoleh pita tunggal untuk masing-masing sampel dengan panjang basa sekitar 700 bp. Kata kunci: gen mtDNA CO1, Ikan Ose, pita DNA, rasio A260/280

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.019

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0010.001
Open science0.0000.001
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0060.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.263
Teacher spread0.241 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2021
Admission routes1
Has abstractyes

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