Cloning, Spatiotemporal Expression and Prokaryotic Expression of ACC Oxidase Gene (<I>BnACO2</i>) in Ramie
Bibliographic record
Abstract
ACC oxidase (ACO) is a key enzyme in ethylene synthesis pathway. This study by rt-pcr with RACE technology get a ACO gene cloning, named BnACO2 , total length of the gene cDNA sequence is 1 377 bp, the open reading frame of 957 bp, coded 318 amino acid polypeptide, predict its molecular weight and isoelectric point (pl) 36.145 kD and 5.60, respectively, bioinformatics analysis showed that BnACO2 no encoding protein signal peptide and transmembrane domain structure, subcellular localization in the cytoplasm. The similarity of ACO gene nucleotide sequence and amino acid sequence was more than 82% and 84% respectively. Phylogenetic analysis showed that the ACO gene was closely related to jute and hemp. Real-time fluorescence quantitative PCR analysis showed that BnACO2 gene was expressed in all parts of ramie, especially in female flower buds. The prokaryotic expression vector PQe- BnACO2 was successfully constructed by double enzyme digestion. At 37°C, 1.0mmol/L IPTG induced PQe- BnACO2 to express the target protein, and the molecular weight of the induced protein was about 36.15kda, which was consistent with the predicted protein size. This lays the foundation for further study on the function of BnACO2 gene.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".