Cloning and prokaryotic expression of the full-length cDNA of CDPK gene from Leymus chinensis
Bibliographic record
Abstract
【Objective】Cloning and prokaryotic expression of CDPK gene from Leymus chinensis would help to further study the function of it,which could probe into its molecule mechanism how L. chinensis adapts itself to the saline-alkali habitat and provide a theoretical basis. 【Method 】 CDPK gene of L. chinensis was cloned by RT-PCR and RACE technology,which was named Lc-CDPK. By constructing prokaryotic expression vector of CDPK gene and transforming it into Escherichia coli BL21( DE3) with IPTG induction,the expression of CDPK protein was analyzed by SDS-PAGE.【Result and conclusion】The full-length c DNA sequence of CDPK gene was 1 704 bp with a 1 647 bp ORF( open reading frame)encoding 548 amino acids,and the amino acids from 81 to 339 formed the S-TKc structural domains possessing catalytic activity of serine/threonine protein kinase and having four conservative EF hand structural domains. A comparison with the nucleotide sequence of coding region of L. chinensis and those of other graminaceous crops showed that it has a close similarity with CDPK gene of wheat,sharing at identity of96%. The relative molecular mass of prokaryotic expressed fusion protein was 61 350 by IPTG induction,which matched up with the anticipated theoretical value. The induced protein reached the highest level at7 hours of induction,accounting for 49. 1% in the total bacterial proteins.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".