Interactions of BLADE-ON-PETIOLE1 and 2 with TALE Homeobox Genes in the Regulation of Flowering and Inflorescence Architecture in Arabidopsis Thaliana
Bibliographic record
Abstract
Plants have evolved a diversity of inflorescence architectures and variations in flowering time to maximize reproductive success within their environment. BLADE-ON-PETIOLE 1, 2 (BOP1/2) are a class of BTB-ankryin transcription factors. BOP activity is concentrated at meristem-organ junctions (“lateral organ boundaries”) where it functions to control the morphology of leaves, flowers, fruits, and inflorescences.Gain-of-function BOP1/2 plants mimic loss-of-function in KNOX and BELL three-amino-acid-loop extension (TALE) homeodomain proteins. BEL1-like (BELL) genes PENNYWISE (PNY) andPOUNDFOOLISH (PNF) are required for Arabidopsis thaliana competence to flower, whereas the KNOTTED1-like HOMEOBOX (KNOX) gene BREVIPEDICELLUS (BP) in conjunction with PNY control internode elongation and stem patterning in inflorescences. In this thesis, I used BOP gain-of-function as an approach to uncover interactions with TALE homeobox genes in regulation of flowering and inflorescence architecture.Firstly, I showed that BP/PNY in stems and PNY/PNF in meristems restrict BOP1/2 expression to boundaries to promote flowering, internode elongation and vascular patterning. Secondly, I identified lateral organ boundary genes KNOTTED-like from ARABIDOPSIS THALIANA6 (KNAT6) and ARABIDOPSIS THALIANA HOMEBOX GENE1 (ATH1) as required by BOP1/2 to antagonize BP/PNY activity through reciprocal regulation of downstream target genes, including biosynthetic enzymes required for lignin deposition in stems. Lastly, BOP1/2 lack a DNA-binding domain and associate with promoter DNA by binding to TGACG (TGA) bZIP transcription factors. I identified TGA1 and TGA4 bZIP factors required by BOP1/2 to exert changes in flowering and inflorescence architecture.Collectively, these findings shed light on how interplay between BOP1/2 and KNOX-BELL complexes in the meristem and lateral organ boundaries governs flowering and inflorescence architecture in a model plant species.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".