Identification of Calpain 10 Isoforms (b, d, e, f & h) Conserved Regions and Possible Functional Prophecy through Bioinformatics
Bibliographic record
Abstract
Calpain 10 is an atypical calpain ubiquitously exist in all human tissues. It exhibits eight protein isoforms designated as “a-h” which play a vital role in glucose homeostasis but actual mechanism of action is yet to be ascertained. We have predicted the partial roles of Isoform a, c and g previously. They were envisaged to act partially as mu and m-calpain cysteine proteases. Here we predict the function of minor isoforms b, d, e, f and h. We have applied NCBI Blast and Conserved domain tool for nucleotide and protein alignments. Blast query indicated 87%, 84%, 87%, 94% and 34% identity of isoform b, d, e, f and h with canonical sequence of calpain 10 a isoform. Conserved domain analyses of protein sequences revealed significant structural similarities of their N-terminal domain I and II with catalytic domain of cysteine protease superfamily PC1 (e-value:CAPN10b, d, e = 2.41e-76, CAPN10f = 1.07e-43 and CAPN10h = 1.13e-17). Isoform b, d and e have one consecutive domain similar with C2 like subdomain III (e-value=2.92-32, 1.03e-35, 1.88e-14 respectively) and was classified in CAPN10 group of Palb subfamily. Isoform f and h were lacking this domain and had shorter sequences. Although structural similarities are not guaranteed for similar actions but domain homology predicted the existence of similar functions as of calpain I and II.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.005 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".