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Record W3153122599 · doi:10.24908/iqurcp.10734

Why is that Plant Bald? A Study of pAG51 mutant Arabidopsis thaliania

2018· article· en· W3153122599 on OpenAlexvenueno aff
Evelyn Popiel

Bibliographic record

VenueInquiry Queen s Undergraduate Research Conference Proceedings · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant Reproductive Biology
Canadian institutionsnot available
Fundersnot available
KeywordsTrichomeMutantBiologyArabidopsisArabidopsis thalianaGeneGeneticsPhenotypeGenetic screenCandidate geneMutationReverse geneticsCell biologyBotany

Abstract

fetched live from OpenAlex

Trichomes, the unicellular hair-like projections found on the leaves of plants, present an ideal system for the study of cell fate and morphogenesis for biologists. They are easily accessible on the epidermis of plant leaves, they are good candidates for genetic manipulation, and they display easily identifiable mutant phenotypes. In addition to their utility as a model study system, trichomes have a variety of important functions in plants, including defence against insects and pathogens, reduction of water loss, and increased resistance to stress. This study will attempt to further characterize the trichome development pathway through the study of a mutant line of Arabidopsis thaliana that does not develop trichomes on its leaves. I hypothesize that one of the genes near the mutation site in pAG51 has decreased expression, and that this is the cause of the mutant’s trichome-less appearance. To test my hypothesis, I will first assess the transcript levels of the genes near the mutation site using a technique called Quantitative Polymerase Chain Reaction (QPCR). Once a gene has been identified, its function will be tested in vivo. The candidate gene will be knocked out in wild type A. thaliana, and if the progeny of this plant grow without trichomes, this will confirm the function of the candidate gene in trichome formation. Finally mutant pAG51 will be bred with another line of mutant A. thaliana, and the progeny phenotypes will be observed to understand how the candidate gene interacts with other genes in the trichome initiation pathway.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.044
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.111
GPT teacher head0.362
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2018
Admission routes1
Has abstractyes

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