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Record W3154785933 · doi:10.1016/j.cub.2021.04.027

Environmental genomics of Late Pleistocene black bears and giant short-faced bears

2021· article· en· W3154785933 on OpenAlexaffabout
Mikkel Winther Pedersen, Bianca De Sanctis, Nedda F. Saremi, Martin Sikora, Emily E. Puckett, Zhenquan Gu, Katherine L. Moon, Joshua D. Kapp, Lasse Vinner, Zaruhi Vardanyan, Ciprian F. Ardelean, Joaquín Arroyo‐Cabrales, James A. Cahill, Peter D. Heintzman, Grant D. Zazula, R. D. E. MacPhee, Beth Shapiro, Richard Durbin, Eske Willerslev

Bibliographic record

VenueCurrent Biology · 2021
Typearticle
Languageen
FieldEnvironmental Science
TopicWildlife Ecology and Conservation
Canadian institutionsYukon Department of Tourism and CultureUniversity of Toronto
FundersNovo Nordisk FondenCarlsbergfondetDeutsche ForschungsgemeinschaftNatural Resources, Energy and Science Authority of Sri LankaWellcome TrustGordon and Betty Moore FoundationH. Lundbeck A/SLundbeckfondenNational Science Foundation
KeywordsBiologyGenomicsEvolutionary biologyPleistoceneZoologyPaleontologyGeneticsGenomeGene

Abstract

fetched live from OpenAlex

Analysis of ancient environmental DNA (eDNA) has revolutionized our ability to describe biological communities in space and time, 1–3 by allowing for parallel sequencing of DNA from all trophic levels. 4–8 However, because environmental samples contain sparse and fragmented data from multiple individuals, and often contain closely related species, 9 the field of ancient eDNA has so far been limited to organellar genomes in its contribution to population and phylogenetic studies. 5 , 6 , 10 , 11 This is in contrast to data from fossils 12 , 13 where full-genome studies are routine, despite these being rare and their destruction for sequencing undesirable. 14–16 Here, we report the retrieval of three low-coverage (0.03×) environmental genomes from American black bear ( Ursus americanus ) and a 0.04× environmental genome of the extinct giant short-faced bear ( Arctodus simus ) from cave sediment samples from northern Mexico dated to 16–14 thousand calibrated years before present (cal kyr BP), which we contextualize with a new high-coverage (26×) and two lower-coverage giant short-faced bear genomes obtained from fossils recovered from Yukon Territory, Canada, which date to ∼22–50 cal kyr BP. We show that the Late Pleistocene black bear population in Mexico is ancestrally related to the present-day Eastern American black bear population, and that the extinct giant short-faced bears present in Mexico were deeply divergent from the earlier Beringian population. Our findings demonstrate the ability to separately analyze genomic-scale DNA sequences of closely related species co-preserved in environmental samples, which brings the use of ancient eDNA into the era of population genomics and phylogenetics.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.238
Teacher spread0.220 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations86
Published2021
Admission routes2
Has abstractyes

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