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Record W3160674833 · doi:10.1038/s42003-021-02108-y

Collagen analogs with phosphorylcholine are inflammation-suppressing scaffolds for corneal regeneration from alkali burns in mini-pigs

2021· article· en· W3160674833 on OpenAlexafffund
Fiona C. Simpson, Christopher D. McTiernan, Mohammad Mirazul Islam, Oleksiy Buznyk, Philip N. Lewis, Keith M. Meek, Michel Haagdorens, Cindy Audiger, Sylvie Lesage, François-Xavier Gueriot, Isabelle Brunette, Marie-Claude Robert, David R. Olsen, Laura Koivusalo, Aneta Liszka, Per Fagerholm, Miguel González‐Andrades, May Griffith

Bibliographic record

VenueCommunications Biology · 2021
Typearticle
Languageen
FieldMedicine
TopicCorneal Surgery and Treatments
Canadian institutionsUniversity of OttawaMcGill UniversityCentre Hospitalier de l’Université de MontréalUniversité de MontréalHôpital Maisonneuve-Rosemont
FundersEuropean Social FundEuropean Regional Development FundInstituto de Salud Carlos IIINatural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchUniversité de MontréalLinköpings UniversitetAgence Nationale de la RechercheDepartment of Biotechnology, Ministry of Science and Technology, IndiaMedical Research CouncilCardiff UniversityUppsala UniversitetGovernment of CanadaUniversity of Ottawa
KeywordsCorneaPhosphorylcholineBiomaterialPEG ratioInflammationRegeneration (biology)ChemistryCorneal inflammationPolyethylene glycolKeratoprosthesisBiomedical engineeringMedicineOphthalmologyCell biologyImmunologyBiochemistryBiology

Abstract

fetched live from OpenAlex

The long-term survival of biomaterial implants is often hampered by surgery-induced inflammation that can lead to graft failure. Considering that most corneas receiving grafts are either pathological or inflamed before implantation, the risk of rejection is heightened. Here, we show that bioengineered, fully synthetic, and robust corneal implants can be manufactured from a collagen analog (collagen-like peptide-polyethylene glycol hybrid, CLP-PEG) and inflammation-suppressing polymeric 2-methacryloyloxyethyl phosphorylcholine (MPC) when stabilized with the triazine-based crosslinker 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. The resulting CLP-PEG-MPC implants led to reduced corneal swelling, haze, and neovascularization in comparison to CLP-PEG only implants when grafted into a mini-pig cornea alkali burn model of inflammation over 12 months. Implants incorporating MPC allowed for faster nerve regeneration and recovery of corneal sensation. CLP-PEG-MPC implants appear to be at a more advanced stage of regeneration than the CLP-PEG only implants, as evidenced by the presence of higher amounts of cornea-specific type V collagen, and a corresponding decrease in the presence of extracellular vesicles and exosomes in the corneal stroma, in keeping with the amounts present in healthy, unoperated corneas.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.240
Threshold uncertainty score0.471

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.052
GPT teacher head0.322
Teacher spread0.270 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations33
Published2021
Admission routes2
Has abstractyes

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