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STRUCTURE ‐ FUNCTION ANALYSIS OF THE LIGAND BINDING POCKET OF BITTER TASTE RECEPTOR T2R4

2013· article· en· W3166768313 on OpenAlexaff
Sai P. Pydi, Ken M. Nelson, Michèle C. Loewen, Rajinder P. Bhullar, Prashen Chelikani

Bibliographic record

VenueThe FASEB Journal · 2013
Typearticle
Languageen
FieldNursing
TopicBiochemical Analysis and Sensing Techniques
Canadian institutionsPlant Biotechnology InstituteUniversity of Manitoba
Fundersnot available
KeywordsChemistryReceptorBitter tasteContext (archaeology)BiochemistryLigand (biochemistry)G protein-coupled receptorStereochemistryAbscisic acidHEK 293 cellsTasteBiologyGene

Abstract

fetched live from OpenAlex

Bitter taste receptors (T2Rs) belong to the G protein coupled receptors super family. The 25 human T2Rs are activated by wide range of bitter compounds which includes alkaloids, terpenoids, peptides and amines. The human T2R4 is activated by around 16 bitter agonists, which are mostly sesquiterpene lactones, amine derivatives, purine analogs and aromatic quinolines. From molecular modeling analysis we found that, the plant hormone abscisic acid (ABA), an ABA biosynthetic precursor and few of the ABA metabolites docked with high affinity to T2R4. The ability of these compounds to activate or inactivate T2R4 was analyzed by calcium imaging in a HEK293 cell based assay system. We discuss the results in the context of T2R4 structure and function.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.222
Teacher spread0.212 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2013
Admission routes1
Has abstractyes

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