Identification and characterization of a NADPH oxidase target in Fusarium graminearum
Bibliographic record
Abstract
Fusarium graminearum is a fungal plant pathogen that causes Fusarium Head Blight (FHB) on important food and feed cereal crops including wheat, maize and barley.Earlier studies identified distinct roles for NADPH oxidase (NOX) genes in F. graminearum.NOX enzymes generate reactive oxygen species (ROS) including hydrogen peroxide (H 2 O 2 ), which are important in signal transduction.To elucidate the mechanism of NOX dependent signaling in pathogenicity, a proteomics approach was used to examine redox changes in the ΔnoxA/B mutant and compared to wildtype F. graminearum strain.Candidate substrates of NOX enzymes were characterized by genetic analysis.Deletion and overexpression of one of the candidate genes, FGSG_10089 with modified cysteine residues confirmed that it is likely a genuine substrate of the NOX enzyme complex.Bioinformatics and expression analysis indicate that this protein may function as a virulence factor.Deletion of FGSG_10089 as well as modification of the cysteine residue C 325 resulted in reduced virulence on wheat.In addition, there was a decrease in production of 15-acetyl deoxynivalenol in culture.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".