Pch2 is a meiotic hexameric ATPase that binds to and alters Hop1 functions
Bibliographic record
Abstract
In most organisms, the segregation of chromosomes during the first meiotic division requires at least one crossover between pairs of homologous chromosomes. Crossovers come from programmed double‐strand breaks (DSBs) that are preferentially repaired using the homologous chromosome as a template (this preference is called interhomolog bias). In budding yeast, PCH2 is required to establish proper meiotic chromosome structure, regulate timing of recombination progression and promote interhomolog bias. How Pch2 functions at the molecular level is unknown despite its wide conservation. PCH2 open reading frame contains regions homologous to AAA ATPase proteins, and here we show that: 1. Pch2 is an ATPase that forms a hexamer in the presence of nucleotides. 2. Both ATP binding and hydrolysis by Pch2 are critical for its in vivo functions. 3. Pch2 binds to Hop1, an axial component of the synaptonemal complex, and alters its DNA binding activity. Based on these and previous cell biological observations, we propose that Pch2 acts as a stringency factor in meiosis to facilitate Hop1 binding to specific chromosomal regions, thus licensing steps that monitor recombination progression and lead to interhomolog bias. C.C. and E.A. are supported by NIH GM53085. J.O. is supported by Canadian Institutes of Health Research (MOP‐82930). A.J. is supported by a CIHR Doctoral Research Award.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".