Vitro seedling development and Asymbiotic seed germination of Epidendrum ibaguense Kunth. (Orchidaceae)
Bibliographic record
Abstract
Effects of four asymbiotic media; Murashige and Skoog (MS), Phytamax (Sigma, USA; PM), Mitra et al. (M) and Knudson ‘C’ (KC) were evaluated for seed germination and early protocorm development in Epidendrum ibaguense Kunth. Besides, effects of peptone, activated charcoal and plant growth regulators (6-benzylaminopurine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D)) were also studied. Maximum seed germination was achieved on M and PM (~90%) fortified with 2 gl-1 peptone. Medium supplemented with 1.0 mgl-1 BAP improved seed germination in contrast to basal media whereas 2,4-D either singly or in combination with BAP remarkably reduced the germination rate. Activated charcoal in the medium enhanced germination of seed, induced significantly large size protocorms (1.63�?�18) and early rooting in protocorms. Within two weeks of culture, spherules emerged out due to cracking of the seed coat. The spherules developed into protocorms with a leaf primordium at apical portion after 3 to 4 weeks and gradually produced complete seedlings. Strong and stout root system was induced in in vitro seedlings on transferring in half strength PM and M media fortified with 0.5-1.0 mgl-1 indole acetic acid (IAA). Well-rooted seedlings were transferred to green house with 90% survival.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".