Investigation of the pre‐sensor 1 hairpin in minichromosome maintenance proteins 2–7.
Bibliographic record
Abstract
The minichromosome maintenance proteins 2–7 (Mcm2–7) assemble into the replicative helicase in eukaryotes. To understand how this ring‐shaped helicase translocates along DNA to unwind the duplex nucleic acid, we have analyzed the structure/function relationships of the S. cerevisiae proteins. A pre‐sensor 1 (PS1) hairpin found in archaeal MCMs is thought to be involved in DNA translocation, with a conserved lysine residue predicted to contact the phosphate backbone. We mutated the analogous lysines to alanines in the putative PS1 hairpins of each of the yeast Mcm2–7 subunits. Mutation of the Mcm3 hairpin results in a loss of cellular viability. Its overexpression in the context of a wild‐type allele results in enlarged cells and a reduced growth rate. Purified Mcm2–7 complex containing the Mcm3 hairpin mutant does not unwind DNA in vitro, yet retains wild‐type levels of ATPase activity. Interestingly, individual mutation of the PS1 hairpins of the other subunits does not affect viability. However, these strains do have growth‐related sensitivity to genotoxic stress agents, and cold. Collectively, our data indicates the importance of the PS1 hairpin for Mcm2–7 function. Support from the CIHR
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".