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Global REACH 2018: heightened α‐adrenergic signaling restrains blood flow to precisely match oxygen delivery and demand during handgrip exercise in Andeans with polycythemia

2021· article· en· W3171200123 on OpenAlexaff
Alexander B. Hansen, Gilbert Moralez, Sachin B. Amin, Florian Hofstätter, Christopher Gasho, Michael M. Tymko, Philip N. Ainslie, Christopher M. Hearon, Justin S. Lawley

Bibliographic record

VenueThe FASEB Journal · 2021
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicHigh Altitude and Hypoxia
Canadian institutionsUniversity of British Columbia, Okanagan CampusUniversity of British ColumbiaUniversity of Alberta
Fundersnot available
KeywordsMedicineInternal medicineCardiologyForearmSurgery

Abstract

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Introduction Populations suffering from polycythemia such as polycythemia vera, Chuvash polycythemia, and chronic mountain sickness (CMS) suffer exercise fatigue and reduced exercise capacity. Potential mechanisms for reduced exercise capacity include the negative influences of high hematocrit, elevated blood viscosity, and increased sympathetic activity on convective and diffusive oxygen transport. However, it remains unclear how sympathetic activity impacts blood flow during isolated forearm exercise in Andeans suffering with polycythemia. Purpose Therefore, using the isolated forearm handgrip model, the present study sought to examine the role of α‐adrenergic vasoconstrictor signaling in regulating skeletal muscle vasodilator function in seven Andeans with polycythemia (CMS+; n=7) and nine of their healthy townsmen (CMS‐; n=9) living at ~4350m. Methods Forearm blood flow (FBF; Doppler ultrasound), mean arterial pressure (MAP, intra‐arterial catheter), and changes in forearm vascular conductance (∆FVC), calculated via FBF and MAP, were measured at rest and during graded handgrip exercise (HGE; 5, 15, 25% maximal voluntary contraction; MVC), before and after local +α‐β adrenergic blockade (phentolamine; propranolol). Blood samples were taken via arterial and venous catheter at rest and each stage of handgrip exercise to determine oxygen delivery, uptake, and extraction. All data are quantified as mean±SD. Results During the control trial, progressive HGE increased FVC (CMS‐: P<0.01; CMS+: P<0.01), oxygen delivery (CMS‐: P<0.01; CMS+: P<0.01) and extraction (CMS‐: P<0.01; CMS+: P<0.01) similarly in both CMS‐ and CMS+ Andeans. However, after +α‐β adrenergic blockade, the FVC response to HGE at 25% was greater in CMS+ (∆FVC: control: 92.8±31.1 vs. +α‐β blockade: 137.5±66.7 ml·min ‐1 ·100mmHg ‐1 ; P<0.01) whereas the FVC response remained unchanged in CMS‐ (∆FVC: control: 109.0±32.8 vs. +α‐β blockade: 95.5±57.9 ml·min ‐1 ·100mmHg ‐1 ; P=0.15). In CMS+, increased FVC during +α‐β adrenergic blockade also increased oxygen delivery during 25% HGE (∆DO 2 : control: 21.9±5.9 vs. +α‐β blockade: 29.7±9.9 ml·min ‐1 ; P<0.01); however, oxygen extraction remained unchanged (∆a‐VO 2 : control: 9.6±4.7 vs. +α‐β blockade: 8.3±4.1 ml·dl ‐1 ; P=0.46). Conclusion CMS+ individuals demonstrate heightened α‐adrenergic restraint of vasodilation during HGE. However, oxygen uptake in the forearm during exercise remains intact due to elevated arterial oxygen content from increased hemoglobin. Perhaps, a phenotypical trade‐off to polycythemia; whereby heightened vasoconstrictor signaling restrains blood flow to match the needed metabolic demand, or elevated whole blood viscosity elicits greater shear signaling requiring higher sympathetic restraint.

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How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.211
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2021
Admission routes1
Has abstractyes

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Same venueThe FASEB JournalSame topicHigh Altitude and HypoxiaFrench-language works237,207