Modulation of αENaC mRNA expression under different stresses: Implication of its 3′UTR.
Bibliographic record
Abstract
Active sodium transport by the epithelial Na+ channel (ENaC) is important for fluid movement across the alveolar epithelium in the lungs and for the resolution of pulmonary oedema in acute respiratory distress syndrome (ARDS). It was shown that cellular stress induced by lipopolysaccharides (LPS) or cycloheximide (Chx) downregulate αENaC mRNA via different molecular mechanisms. The purpose of this project was to investigate the importance of the αENaC 3′UTR in the mRNA modulation of ENaC. Alveolar epithelial cells were co‐transfected with 3′UTR mutants (V5/ENaC±3′UTR and Luc±3′UTR) inserted in pTRE‐tight vector along with the Tet‐Off vector. The cells were treated with Chx (1.0μM), LPS (15μg/mL) or Actinomycin D (5μg/mL) along with Dox (1μg/mL) to inhibit transcription. We found that 3′UTR deletion caused a ~60% decrease of luciferase activity and expression compared to the complete recombinant. The T 1/2 of V5‐αENaC mRNA was 60 min and the deletion of 3′UTR reduced T 1/2 to 37min. Chx reduced αENaC mRNA T 1/2 to 33min whereas LPS had no effect. Actinomycin D, a transcription inhibitor, increased T 1/2 >; 120min. These results suggest that the αENaC mRNA half‐life is shorter then previously estimated and that 3′UTR seems to be important for the stability of αENaC mRNA. Supported by FRSQ, CFC and CIHR.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".