An isoform of actin is associated with the cancer status of ovarian and breast epithelial cells
Bibliographic record
Abstract
An isoform of actin associated with non‐malignant ovarian and breast epithelial cells was detected in a study investigating cancer specific forms of PCNA (proliferating cell nuclear antigen) in ovarian and breast cancer materials. An antibody (B1 antibody) generated against ten residues from the interdomain connector loop in PCNA, detected not only PCNA, but a novel protein in non‐malignant cells. Immunoprecipitation was performed using the B1 antibody and proteins were cut from gels. In‐gel trypsin digestion was performed for MALDI‐TOF analysis. The novel protein was identified as a form of actin using Mascot peptide fingerprinting software. Immunoblotting with actin antibodies support the identification of the B1 protein as an isoform of actin. A local alignment between PCNA and beta‐actin was performed and found a ten‐residue area of overlap with 50% homology between PCNA and beta‐actin which corresponded to the antigen for the B1 antibody. This homology is likely the basis of the cross reaction with beta‐actin. To avoid the cross reaction with PCNA exhibited by the B1 antibody, a second antibody (ACTB358) was generated to 11 residues starting at residue 358 of beta actin The ACTB358 antibody is highly specific for a novel isoform of actin with a mass of 86 kDa and a pI of 5.5, but does not interact with cytoplasmic or other types of actin. We are investigating the sequence and expression of the novel isoform of actin in non‐malignant and malignant ovarian and breast cells. Support by NOSMFA Research Development Fund
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".