Alterations in mitochondrial fission and fusion proteins with chronic muscle use and disuse
Bibliographic record
Abstract
The maintenance of mitochondrial shape depends on reaching an equilibrium between fission and fusion. To investigate the role of fission and fusion regulatory proteins in mitochondrial biogenesis, the content of these proteins was evaluated in mitochondrial fractions and whole muscle of animals subjected to chronic muscle use and disuse. The tibialis anterior (TA) muscle of rats was subjected to either chronic contractile activity (CCA, 10Hz, 3 hours/day, 7 days) or denervation for 7 days. The contralateral limb of both sets of animals served as internal controls. With chronic disuse, the fission proteins Fis1 and Drp1 decreased by 1.9 and 1.3-fold, respectively. Furthermore, the expression of the fusion proteins Opa1 and Mfn2 was reduced in mitochondria by 6.3 and 4.2-fold, respectively. CCA had the opposite effects on the proteins governing mitochondria morphology, as 1.4 and 2.0-fold increases in Opa1 and Mfn2 were observed. However, Drp1 was unaffected by CCA, while Fis1 was increased by 1.5-fold. Thus, mitochondrial biogenesis appears to be associated with increases in the ratio of fusion:fission proteins, while the opposite is evident with muscle disuse. Supported by NSERC
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".