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Regional Adipose Tissue Immune Cell Profiles in Childhood‐Onset and Adult‐Onset Obesity

2018· article· en· W3173828419 on OpenAlexafffundabout
Jessica Murphy, Vi Dam, Kerri Z. Delaney, José A. Morais, Michael A. Tsoukas, Sylvia Santosa

Bibliographic record

VenueThe FASEB Journal · 2018
Typearticle
Languageen
FieldMedicine
TopicAdipokines, Inflammation, and Metabolic Diseases
Canadian institutionsMcGill UniversityMcGill University Health CentreConcordia University
FundersNatural Sciences and Engineering Research Council of CanadaHeart and Stroke Foundation of Canada
KeywordsAdipose tissueImmune systemObesityCD8Internal medicineChildhood obesityMedicineEndocrinologyBiologyImmunologyOverweight

Abstract

fetched live from OpenAlex

Background We do not understand why individuals who have had obesity since childhood are at greater risk of metabolic disease. Objective To examine the effects of obesity onset and adipose tissue region (upper vs. lower body) on the proportion of adipose tissue immune cells. Methods We used flow cytometry to quantify the proportion of immune cells in the stromovascular fraction of abdominal (AB) and femoral (TH) subcutaneous adipose tissue from adults with childhood‐onset (n = 16) or adult‐onset (n = 22) obesity. Results The proportion of CD8+CD3+ T‐cells was greater in AB than TH in childhood‐onset obesity, while greater in TH than AB in adult‐onset obesity. There was no effect of obesity onset on other immune cell types. However, there was an overall effect of region where the proportion of CD45RA+CD8+CD3+ T‐cells was greater in TH than AB, and the proportion of CD206+CD68+ M2‐like macrophages was greater in AB than TH. Conclusion Our results show that there are regional differences in the adipose tissue immune cell profiles between childhood‐onset and adult‐onset obesity. It remains to be seen whether these differences are implicated in increasing the risk of metabolic disease. Support or Funding Information This research was funded by NSERC and the Heart and Stroke Foundation of Canada This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.248
Teacher spread0.237 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2018
Admission routes3
Has abstractyes

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