BNIP3 and OGD‐induced oligodendrocyte death: Role in white matter injury in cerebral ischemia
Bibliographic record
Abstract
We sought to identify potential role of BNIP3, a proapoptotic member of the Bcl‐2 family of death‐regulating proteins that promote programmed cell death, in Oxygen–glucose deprivation (OGD) treated primary cultures of rat oligodendrocyte lineage cells (OLs). OGD induced cytotoxic injury to various maturational stages of OLs. Survival assays revealed that oligodendrocyte precursor cells (OPCs) and later‐stage precursors were highly vulnerable, whereas more mature OLs were relatively resistant. Exposure of OLs to OGD resulted in a significant increase in BNIP3 expression. Knockdown of BNIP3 with shRNA reduced OGD‐triggered OL apoptosis. Thirty minutes ischemic preconditioning (IPC) of later‐stage precursors and more mature OLs down‐regulated the BNIP3 expression and shown cytoprotection. Interestingly, OPCs were more susceptible to OGD after IPC pretreatment, concurrent with highly BNIP3 expression. These studies unveil BNIP3 as an important mediator in OLs death and reveal a novel mechanism of action of IPC on stage‐specific OLs cultures, which may contribute to a better understanding of white matter injury in cerebral ischemia.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".